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BIO302 Molecular Biology Assignment No 01 Fall 2019 Solution & Discussion

Q:What is Bacterial Transformation?

Q:Write down the steps of bacterial transformation?

Q:: What are the Application of Bacterial transformation

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BIO302 Assignment no 02 Solution Fall 2019 November 2019

 Assighnment No1 Bio302
Q:What is Bacterial Transformation?

Ans:: Bacterial transformation is a process of horizontal gene transfer by which some bacterial take up foreign genetic material (Naked DNA) from the environment.it was first reported in streptococcus penumoniae by Griffith in 1928.DNA as the transforming principle was demonstrated by Avery in 1944.
The process of Gene transfer by transformation does not require a living donor cell but only requires .The presence of persisstant DNA in the Environment .
The prequisile for bacterial to undergo transformation is its ability to take up free extracellular genetic material such as bacteria termed as competent cells.

Q:Write down the steps of bacterial transformation?

Ans:1)Specially prepared bacteria are mixed with DNA(e.g from ligation)
2) The bacteria are given a heat shock,which encourages them to take up plasmid.
3)Plasmids used in cloning contain an antibiotic resistance gene.
4)Bacteria without a plasmid die.

Q:: What are the Application of Bacterial transformation

Ans:
The phenomenon of transformation has been widely used in molecular biology .As they are easily grown in large number transformed bacteria may be used as host cells for the following to take multiple copies of DNA in cloning procedures to express large amounts of proteins and enzymes in the generation of cDNA libraries in DNA linkage studies.

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