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BT605 GDB Spring 2019 Solution & Discussion

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GDB Idea Sol


Microbiology Laboratory Biosafety Guidelines

Complete the following questions by writing the letter of the correct answer on the line.

___ 1. Biological agents are assigned to Biosafety Levels (BSLs) based on

  1. whether they are bacteria, viruses, or other microorganisms.
  2. their susceptibility to laboratory disinfectants.
  3. the risk they pose to human health and the environment.
  4. the amount of the agent that will be used in the lab.

___ 2. BSL 1 practices, equipment, and facilities are used when working with which type of microorganism?

  1. Bacteria that are well characterized and not known to consistently cause disease in healthy adult humans.
  2. Bacteria that are considered moderate-risk agents present in the community and associated with human diseases.
  3. Indigenous or exotic bacteria with potential for aerosol transmission and associated with human disease.
  4. Dangerous/exotic agents with a high risk of life-threatening disease.

___ 3. The primary hazards to people working in a BSL 1 or 2 laboratory relate to

  1. accidental skin punctures caused by mishandling of sharp lab tools.
  2. mucous membrane exposure due to accidental splashing of cultures.
  3. accidental ingestion of infectious material.
  4. All of the above are primary hazards in a BSL 1 or 2 lab.

___ 4. Which of the following is required when working with BSL 2 agents?

  1. Biohazard warning signage on doors and lab areas where agents are kept or used.
  2. Self-closing, double door access to the lab facility.
  3. Clothing change when entering or leaving the lab.
  4. There are no special precautions needed for a BSL 2 lab.

___ 5. What is the appropriate disposal method for a bacterial culture grown on an agar plate?

  1. Throw the culture away in any one of the several garbage containers in the room.
  2. Throw the culture away in the garbage container marked with a biohazard sign.
  3. Allow the agar to dehydrate in the incubator and then discard in any garbage.
  4. Bacteria grown on an agar plate medium should never be disposed of.

Microbiology Laboratory Biosafety Affirmation

By signing your name below, you affirm that:

Potential hazards in the microbiology laboratory have been identified and explained.

You understand that the bacterial cultures used in lab require either BSL 1 or BSL 2 containment procedures.

Safety practices for BSL 1 and 2 have been explained and/or demonstrated to you.

You fully understand the hazards associated with BSL 1 and 2 bacterial cultures and are willing to assume the risk.

You are responsible for your own actions while working in a laboratory with potentially hazardous materials.



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